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The highly sensitive XF HS Mini analyzer offers improved performance and precision, requiring fewer cells per well, an improved more consistent suspension cell workflow, and streamlined analytics. These metabolic measurements enable researchers to better understand cell health, function, and signaling. The Seahorse XF Cell Mito Stress Test kit is an optimized solution for assessing mitochondrial function. The XF HS Mini is the latest addition to Agilent’s range of Seahorse XF platforms, which analyze mitochondrial respiration, glycolysis, and ATP production in live cells, in real-time. Rotenone is an odorless, colorless, crystalline isoflavone used as a broad-spectrum insecticide, piscicide, and pesticide.It occurs naturally in the seeds and stems of several plants, such as the jicama vine plant, and the roots of several members of Fabaceae.It was the first described member of the family of chemical compounds known as rotenoids. Limitations in the quantities of these cells that they can produce, however, restrict these researchers in the types of cellular assays they can perform. Immunology and disease researchers are increasingly using rare, ex vivo, and genetically engineered cells to build better disease models. at the optimized cell density for each of the different cell lines in Seahorse. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques.
#Difference between seahorse xf and xfe how to#
Here, using the Seahorse XF(e) 24 Extracellular Flux Analyzer and the pharmacological inhibitors dicyclohexylcarbodiimide (DCCD, ATP synthase inhibitor), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP, mitochondrial uncoupler), and sodium azide (cytochrome c oxidase inhibitor), we describe how to obtain in vivo measurements of the fundamental parameters of the mitochondrial respiratory chain in the model organism Caenorhabditis elegans.Enhanced data quality, easier workflow, and more versatile analytics using fewer cell numbers - this is how Agilent sums up the advantages of the new Seahorse XF HS Miniplate. Agilent Seahorse XFe cell mito stress test was performed according to the.
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Polyacrylamide gels were pipetted into the wells (10 mL/well) of a standard XF24 microplate (Seahorse Bioscience, North Billerica, MA) and allowed to crosslink before rinsing with PBS. Because mitochondria function in an organismal milieu, there is need for assays capable of rapidly assessing mitochondrial health in vivo. Cellular metabolism was measured using a Seahorse Bioscience XFe 24 Extracellular Flux Analyzer (North Billerica, MA). This document is intended to be used as a companion to the appropriate Seahorse XF Stress Test Assay Guide or Template. Less well appreciated is the fact that mitochondria integrate environmental and intercellular as well as intracellular signals to modulate function. For Seahorse XF Mito stress test 37☌-preheated XF assay medium was supplemented with 1 mM sodium pyruvate (PAN-Biotech, Aidenbach, Germany), 2 mM L-glutamine and 5 mM glucose (Thermo Fisher Scientific, Waltham, Massachusetts, USA). The Agilent Seahorse Assay Templates and Guides provide cell type-specific protocols for performing both Agilent Seahorse XF Stress Tests on the Agilent Seahorse XFe and XFp Analyzers. Mitochondria are critical for their role in ATP production as well as multiple nonenergetic functions, and mitochondrial dysfunction is causal in myriad human diseases.